Forget rules -- most of the "rules" I've seen published were other people telling me how *I* should use Twitter to enhance *their* experience.
It's simple courtesy. Like what you mentioned about starting tweets with the "@" for webusers. When sending multiple "@"s, I've even been known to see which of the recipients was on the web (instead of twhirl or snitter) so I could line them up first.
Have you been reaching into my notes? Excellent point, and one I haven't seen many places --- this notion that same have that the tools can magically empower people to be instant marketers. Social Media is NOT a commodity.